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| forward pipetting ： ウィキペディア英語版 |
forward pipetting
Forward pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for aqueous solutions, such as buffers, or diluted acids or alkalis. In case of solutions with a high viscosity or a tendency to foam, reverse pipetting is more suitable.
== How it works ==
(詳細はair displacement pipette, the piston inside the instrument moves down to let air out. Air is displaced by the piston. The volume of air displaced is equivalent to the volume of liquid aspirated.
These pipettes are capable of being very precise and accurate. However, since they rely on air displacement, they are subject to inaccuracies caused by the changing environment, particularly temperature and user technique. For these reasons, this equipment must be carefully maintained and calibrated, and users must be trained to exercise correct and consistent technique.
Alternative solutions to improve reproducibility and accuracy of manual pipetting operations are based on liquid handling robots capable of handling air displacement pipettes.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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